The company will also provide program management and systems engineering services as part of the firm-fixed-price and cost-plus-fixed-fee contract, the Defense Department said Wednesday.
The Navy will obligate $147M in fiscal 2018 aircraft procurement funds at the time of award.
Lockheed will perform work in Owego, N.Y., Startford, Conn., and Troy, Ala., through September 2020.
Naval Air Systems Command is the contracting activity.
MH-60R is an anti-surface weapon system and anti-submarine warfare aircraft intended to operate in open ocean and littoral zones.
The helicopter can be flown from aircraft carriers, cruisers, frigates and destroyers.